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                                  小鼠8羟基脱氧鸟苷,小鼠过氧化脂质ELISA试剂盒引用文献
                                  时间:2022-02-15 点击次数:60

                                  【文献标题】Reparative effects of lycium barbarum polysaccharide on mouse ovarian injuries induced by repeated superovulation

                                  【作者】Bing Liu,Jun-Li Wang,Xin-Mei Wang,et.al

                                  【作者单位】北京农业大学(Beijing University of Agriculture)

                                  【文献中引用产品】

                                  小鼠8羟基脱氧鸟苷(8-OHdG)ELISA试剂盒

                                  小鼠过氧化脂质(LPO)ELISA试剂盒

                                  【关键词】Superovulatory times,Injury model,LBP,Remedy,Oxidative stress

                                  【DOI】doi.org/10.1016/j.theriogenology.2020.01.048

                                  【出版期刊】《Theriogenology》

                                  【产品原文引用】

                                  8-OHdG and LPO levels of the collected ovarian tissues were determined using an ELISA kit, according to the manu-facturer’s instructions (Shanghai Hengyuan Biotechnology Co., Ltd,Shanghai, China). The collected mouse ovaries were cracked with lysate. Each 10 mL henylmethane-sulfonyl (PMSF) was added in 1 mL radio immuno precipitation assay (RIPA) as lysate. Every 1 g of tissue with 9 mL lysate, then coated with mouse antibody against 8-OHdG or LPO. After incubation and washing concentrations of 8-OHdG or LPO were determined as described previously .Intra-and inter-CVs: 8-OHdG (6.3%, 8.1%), LPO (5.2%, 7.4%).



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